重组腺病毒(AdV)

 

        重组腺病毒(AdV)是一种复制缺陷的腺病毒载体系统,在基因治疗、基础生命科学研究等领域被广泛应用。目前常用的腺病毒载体基于人腺病毒5型(Ad5),其基因组是36 kb长的线性双链DNA。腺病毒可通过自身的纤维(fiber)和细胞表面的受体结合被内吞进入细胞,然后从内吞体(endosome)转移到细胞质和细胞核内,借助细胞的转录和翻译机器启动病毒的复制组装。一个完整的病毒生活周期会引发细胞死亡从而释放出病毒粒子。

 

重组腺病毒具有以下几个显著优点:

 

感染范围广,几乎可以感染所有的分裂和不分裂细胞系、原代细胞和部分组织;

感染效率高达 100%,可全面超越其他病毒载体工具和脂质体转染;

对外源基因容载能力大(可以高达 8 Kb);

不整合基因组;

滴度高,操作方便。

因此,重组腺病毒是一种最具有潜力的基因递送工具。

 

 

产品特点

 

● 采用AdMAX包装系统,病毒滴度更高;

● E1和E3的缺陷(ΔE1, ΔE3),复制缺陷株病毒,保障操作安全;

● 庞大的腺病毒载体现货库和腺病毒现货库,覆盖绝大部分热门基因,价格更低,周期更短。

 

产品分类

 

● 普通Ad5重组腺病毒-适用普通细胞系感染;

● 原代细胞专用腺病毒-适用各种原代细胞基因转导;

● 悬浮细胞专用腺病毒-专为悬浮细胞特殊设计,也兼容普通细胞和原代细胞,效率更高;

 

感染图谱

 

 

 

参考文献节选

 

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