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Lentivirus Packaging

Lentivirus vector is a gene therapy vector developed on the base of HIV-1 (human immunodeficiency virus type 1). It is a powerful tool for introduction of exogenous genes, with the advantages of wide infection spectrum, effective infection of cell in both dividing and stationary phases, long-term stable expression of exogenous genes. Now the lentivirus system has been widely used in various cell lines for gene overexpression, RNA interference, microRNA research, as well as in vivo animal experiments.

Lentivirus have the following characteristics

Wide range of infection

Lentivirus can effectively infect dividing and non dividing cells, and is suitable for almost all cell lines, such as neuron cells, hepatocytes, myocardial cells, tumor cells, endothelial cells, stem cells, etc.

Stable expression

Lentivirus can integrate foreign genes into the host cell genome without losing them during cell division and passage, achieving long-term stable expression of target genes.

High operational safety

The lentivirus uses a self inactivated replication deficient virus strain to ensure operational safety.

HANBIO provides services related to lentivirus

1. Lentivirus packaging with overexpression and repression of conventional genes.

2. miRNA spone/antago lentivirus.

3. Overexpression and repression of circRNA/lncRNA with lentivirus.

4. Customization of lentivirus for CRISPR/Cas9.

5. Spot stock of lentivirus.

6. Establishing stable cell lines.

HANBIO provides products

Product	Virus Titer	Scope of Application
Conventional lentivirus	10⁸TU/ml	Ordinary cell lines
High titer lentivirus	10⁹TU/ml	Difficult to transfect cell lines, such as Suspension Cultured Cells, or used for animal infection

Advantages of HANBIO lentivirus

1. Low cytotoxicity (purification process using ultracentrifugation)

2. No contamination by Mycoplasma

3. No endotoxin, exogenous microorganisms, and residual host nucleic acid

4. The titer can reach up to 10⁹TU/mL

5. A strong customer centric after-sales service system

6. Massive lentivirus inventory

Lentivirus Vectors

NO.	Regulation	Vector Name	Prokaryotic Antibiotic	Eukaryotic Antibiotic	Fluorescent Label	Promoter
LV005	OE	pHBLV-CMV-MCS-EF1-mCherry-T2A-Puromycin	Ampicillin	Puromycin	mCherry	CMV
LV006	OE	pHBLV-CMV-MCS-3flag-EF1-mCherry-T2A-Puromycin	Ampicillin	Puromycin	mCherry	CMV
LV007	OE	pHBLV-CMV-MCS-EF1-Puromycin	Ampicillin	Puromycin	NONE	CMV
LV008	OE	pHBLV-CMV-MCS-3flag-EF1-Puromycin	Ampicillin	Puromycin	NONE	CMV
LV011	OE	pHBLV-CMV-MCS-3flag-EF1-ZsGreen-T2A-Puromycin	Ampicillin	Puromycin	ZsGreen	CMV
LV012	OE	pHBLV-CMV-MCS-EF1-ZsGreen-T2A-Puromycin	Ampicillin	Puromycin	ZsGreen	CMV
LV052	OE	pHBLV-CMV-MCS-EF1-ZsGreen	Ampicillin	NONE	ZsGreen	CMV
LV05	OE	pHBLV-CMV-MCS-3flag-EF1-ZsGreen	Ampicillin	NONE	ZsGreen	CMV
LV081	OE	pHBLV-EF1-MCS-CMV-Puromycin	Ampicillin	Puromycin	NONE	EF1
LV082	OE	pHBLV-EF1-MCS-CMV-ZsGreen	Ampicillin	NONE	ZsGreen	EF1
LV083	OE	pHBLV-EF1-MCS-CMV-ZsGreen-T2A-Puromycin	Ampicillin	Puromycin	ZsGreen	EF1
LV121	OE	pHBLV-CMV-MCS-3flag-EF1-Luc-T2A-Puromycin	Ampicillin	Puromycin	Luciferase	CMV
LV122	OE	pHBLV-CMV-MCS-EF1-Luc-T2A-Puromycin	Ampicillin	Puromycin	Luciferase	CMV
LV027	OE-teton	pHBLV-TetOn-SV40-Puromycin-TRE3GS-MCS	Ampicillin	Puromycin	NONE	TRE3GS
LV019	RNAi	pHBLV-U6-MCS-PGK-Puromycin	Ampicillin	Puromycin	NONE	U6
LV020	RNAi	pHBLV-U6-MCS-CMV-ZsGreen	Ampicillin	NONE	ZsGreen	U6
LV021	RNAi	pHBLV-U6-MCS-CMV-ZsGreen-PGK-Puromycin	Ampicillin	Puromycin	ZsGreen	U6
LV051	RNAi	pHBLV-U6-MCS-EF1-mCherry-T2A-Puromycin	Ampicillin	Puromycin	mCherry	U6
LV124	RNAi	pHBLV-U6-MCS-EF1-Luc-T2A-Puromycin	Ampicillin	Puromycin	Luciferase	U6
LV144	RNAi	PHBLV-U6-MCS-CMV-mCherry	Ampicillin	NONE	mCherry	U6
LV050	cas9/gRNA	pHBLV-U6-gRNA-EF1-CAS9-Puromycin	Ampicillin	Puromycin	NONE	U6
LV055	cas9/gRNA	pHBLV-U6-gRNA-EF1-ZsGreen	Ampicillin	NONE	ZsGreen	U6
LV133	cas9/gRNA	pHBLV-U6-gRNA-EF1-ZsGreen-Luc	Ampicillin	NONE	Luciferase	U6

Examples of Lentivirus infections

Selected literature published by researchers

T lymphocyte membrane-decorated epigeneticnanoinducer of interferons for cancerimmunotherapy

（Nature Nanotechnology，IF=39.213，Shanghai Institute of Materia Medica）

The circRNA circSEPT9 mediated by E2F1 and EIF4A3 facilitates the carcinogenesis and development of triple-negative breast cancer

（Molecular Cancer，IF=27.401，ChongQing Medical University）

CircPSMC3 suppresses the proliferation and metastasis of gastric cancer by acting as a competitive endogenous RNA through sponging miR-296-5p

（Molecular Cancer，IF=27.401，Nanjing First Hospital, Nanjing Medical University）

Targeting E2 ubiquitin-conjugating enzyme UbcH5c by small molecule inhibitor suppresses pancreatic cancer growth and metastasis

（Molecular Cancer，IF=27.401，Zhejiang Tumor Hospital）

Circular RNA MTCL1 promotes advanced laryngeal squamous cell carcinoma progression by inhibiting C1QBP ubiquitin degradation and mediating beta-catenin activation pathway

（Molecular Cancer，IF=27.401，The First Afliated Hospital of China Medical University）

Extracellular vesicle‐mediated delivery of circDYM alleviates CUS‐induced depressive‐like behaviours

（Journal of Extracellular Vesicles，IF=25.841，Southeast University）

Exosomes derived from osteogenic tumor activate osteoclast differentiation and concurrently inhibit osteogenesis by transferring COL1A1-targeting miRNA-92a-1-5p

（Journal of Extracellular Vesicles，IF=25.841，Xijing Hospital of the Fourth Military Medical University）

TRIB3 Interacts with Beta-catenin and TCF4 to Increase Stem Cell Features ofColorectal Cancer Stem Cells and Tumorigenesis

（GASTROENTEROLOGY，IF=22.682，Peking Union Medical College Hospital）

HACE1-mediated NRF2 activation causes enhanced malignant phenotypes and decreased radiosensitivity of glioma cells

（Signal Transduction and Targeted Therapy，IF=18.187，Xi’an Jiaotong University）

TRIB3 reduces CD8+ T cell infiltration and induces immune evasion by repressing the STAT1-CXCL10 axis in colorectal cancer

（Science Translational Medicine，IF=17.956，Institue of Materia Medica Chinese Academy of Medical Science）